Genome Center

Head   Oliver Weichenrieder
Lab Managers:   Christa Lanz, Heike Budde
     
Phone:   +49 7071 601-348, +49 7071 601-1446
   

Introduction

The Genome Center Facility and the Department of Molecular Biology (Detlef Weigel) introduced next-generation DNA sequencing to our institute as early as 2007. By now, sequencing-based approaches are crucial for the large majority of our research groups. These include de novo genome assemblies, variant calling, metagenomics, chromatin analysis, full-length and short read transcriptomics, small RNA analysis and single-cell applications from organisms as diverse as vertebrates, insects, worms, plants, brown algae, fungi, ciliates and microbes.

The Genome Center hosts a Sequel II instrument from Pacific Biosciences for high-fidelity single-molecule real-time (SMRT) sequencing of DNA fragments with many thousand nucleotides in length. The facility also operates a HiSeq 3000 platform from Illumina routinely generating paired-end short-read data (2 x 150 nucleotides) from small DNA fragments in the order of 800 gigabases per run. Finally, an Illumina MiSeq sequencer generates variable read lengths for small-scale projects.

The Genome Center assures fast, reliable and high-quality sequencing services and supports the development of new application protocols. The facility trains internal and external scientists in preparing libraries, providing lab-space and assistance with technical equipment for library preparation and quality control.

Publications

Equipment

  • Sequel II, Pacific Biosciences
  • HiSeq 3000 and cBot, Illumina
  • MiSeq, Illumina
  • Chromium, 10x Genomics
  • Femto Pulse, Agilent Technologies
  • Bioanalyzer 2100, Agilent Technologies
  • Sage ELF, Sage Science
  • BluePippin, Sage Science
  • CHEF-DR III, BioRad
  • Megaruptor 2, Diagenode
  • Covaris S2, KBiosciences
  • Qubit 2.0 Fluorimeter, Invitrogen
  • Viaflo 96, Integra